印楝采种育苗及造林技术初步研究

对印楝Y.L07种源在元江试验区采种、育苗及造林技术研究结果表明：7月下旬至9月上旬,印楝果皮由青绿色变为黄色时是最佳采种期;自然状态下,种子发芽力随贮存时间的延长而递减;容器苗造林利于成活;苗木定植时可留干25cm进行截干处理。     

一个山羊Ⅰ型毛角蛋白基因的序列及其在皮肤中的表达

角蛋白家族包含表皮软角蛋白和毛发硬角蛋白，可以分为酸性Ⅰ型和碱性或中性的Ⅱ型角蛋白亚家族。从构建的成年山羊皮肤cDNA文库和ESTs分析中发现了和与绵羊羊毛角蛋白8C1和人Ⅰ型毛发角蛋白相应cDNA序列高度同源的序列，经序列分析证明是一个山羊毛发特异性角蛋白，命名为山羊Ⅰ型毛角蛋白1（gHa1），Gen Bank序列号为AY510110．1。推导的读码框ORF由413氨基酸组成，与绵羊8C1角蛋白的序列一致性为97．8％。原位杂交显示它在皮肤初级和次级毛囊的皮质层有强烈的表达。     

暖季放牧犊牦牛对高寒草甸地区人工草地的影响

为更好地了解青藏高原高寒草甸区暖季放牧条件下人工草地牧草的生长特点,为制定合理放牧制度提供依据,于2001~2003年开展了暖季放牧牦牛对多年生人工草地影响的试验。结果表明:放牧2年后轮牧人工草地播种牧草比例为95.27%,连续放牧人工草地为86.15%,无放牧人工草地为58.01%。轮牧人工草地牧草月风干重最高值3 198.8 kg/hm2,连续放牧人工草地为3 023.2 kg/hm2。放牧可以提高人工草地牧草生长率,轮牧人工草地的牧草生长率高于连续放牧人工草地。放牧还影响着第2年播种牧草的生长,轮牧能促进种子出苗数,播种牧草的鲜重,风干重和种子产量分别达到了5 088.88 kg/hm2,3 053.33 kg/hm2和480 kg/hm2,均高于连续放牧人工草地和无放牧草地,轮牧人工草地杂草鲜草产量最低,为193.33 kg/hm2。适宜的放牧是保持人工草地可持续利用的手段之一。     

鸡贮精腺差异表达基因的研究

用mRNA差异显示技术对鸡贮精腺中基因的差异表达进行研究,分离并筛选出1个差异表达片段SST01,经测序和BLAST分析,发现SST01与鸡2号染色体上的neurexophilin基因高度同源(98%)。Neurex-ophilin是一种只在神经细胞中分泌的糖蛋白,说明神经系统参与贮精腺内精子的贮存或释放。Neurexophilin作为精液素的配体发挥功能,而精液素使细胞粘连,从而说明neurexophilin参与精子在贮精腺内的贮存过程。     

Embryonic stem cells transplant into subretinal space of C3B mouse

AIM:To study the differentiation of embryonic stem cells (ESC) at the subretinal space of C3B mouse whose retina are of normal structure. METHODS:Embryonic bodies (EB) were gained when embryonic stem cells were propagated 1 generation after anabiosis. The digested EB were transplanted into subretinal space of C3B mouse. The eyes were analysed by photo microscopy, electric microscopy and immune assay at 1st week, 3rd week and 2nd month after injection. RESULTS:The thickened retina appeared on the place of injection at 1st week. Teratoma formation was observed in 3 transplant recipients at 3rd week and 2nd month. The ratio of forming tumor was 25% in all eyes received cell transplant. Eye atrophy or scar at injection position was seen in other eyes. The nucleus of tumour cells had apparent strange type under electrical microscopy. The immune assay showed that part of the tumour were microtubule-associated protein 2 (MAP-2) positive;part of the tumour were glial fibrillary acid protein (GFAP) positive;a little cells were nestin positive. CONCLUSION:After transplantation into the subretinal space, ESC were not incorporated into retina or differentiated into retinal cells. The teratoma was formed in part of eyes. The security of application for ESC in eye was considerable.     

Expression of telomerase inhibitor Pinx1 in leukemia cells and its correlation with telomerase activity

AIM:To study the expression of telomerase inhibitor Pinx1 in acute leukemia cells and during the differentiation of acute promyelocytic leukemia cells,and to realize its effect on telomerase activity.METHODS:Realtime quantitative PCR with fluorescence probe hybridization was used to measure the expression of Pinx1 and hTERT mRNA in acute leukemia cells and during differentiation of NB4 cells induced by ATRA.The correlations between Pinx1 and hTERT expression were also analyzed.RESULTS:Pinx1 mRNA expression in acute leukemia samples (0.00312,5.42×10-4-0.024) was significantly higher than that in normal bone marrow mononuclear cells (7.89×10-4,0-0.00863,P<0.01).The expression of Pinx1 mRNA had significant positive correlation with hTERT mRNA expression (r=0.296,P<0.05).Pinx1 mRNA expression decreased during differentiation,its expression was positive correlated with hTERT mRNA expression (r=0.900,P<0.05).CONCLUSIONS:As an inhibitor of telomerase,however,Pinx1 also had the same direction of regulation with telomerase activity in acute leukemia cells,suggesting its expression variation may be a subsequent reaction induced by that of hTERT to stabilize telomerase activity.The exact mechanisms remained to be verified.     

猪繁殖与呼吸综合征活疫苗(CH-1R株)工厂化生产培养条件的研究

为提高猪繁殖与呼吸综合征病毒(PRRSV)活疫苗CH-1R株的产量和疫苗的质量,本实验探索了PRRSV活疫苗CH-1R株在MARC-145细胞中大规模生产培养的最佳条件。将CH-1R株分别在3L和10L转瓶培养的MARC-145细胞中进行增殖试验,分析CH-1R株在不同接种病毒量和不同接种病毒时间的TCID50变化规律。结果表明,在3L和10L转瓶中,病毒的TCID50最高可达到107.0TCID50/mL以上。CH-1R株通过转瓶在MARC-145细胞中大规模增殖,为PRRSVCH-1R株的工厂化生产奠定了基础。     

海南香荚兰产业化发展种植模式探讨

通过介绍香荚兰生长的环境条件、开花结荚习性和国外香荚兰发展的成功经验,分析海南发展香荚兰的利弊因子和现有种植模式的投资概算,探讨一种投资少发展快、效益高的种植模式,以促进海南香荚兰生产的产业化高效发展。     

鲁西黄牛耳缘组织成纤维细胞的体外培养

实验以鲁西黄牛耳缘组织为研究材料,采用组织块贴壁培养法对其进行了成纤维细胞的体外培养。通过细胞的传代培养、形态学和动力学观察与分析,结果表明,所建立的鲁西黄牛耳缘组织成纤维细胞系具有典型的成纤维细胞形态,胞体均为梭形和不规则三角形,且细胞丰满;细胞核形态为卵圆形,并位于胞质中央,核仁清晰。在细胞生长于繁殖过程中亦经历了潜伏期、指数增生、停滞期和衰退死亡期等4个时期。细胞群体倍增时期为72h。实验结果表明,已成功地培养了鲁西黄牛耳缘组织成纤维细胞。